Automated 96-well protein quantification With the Nano-Glo® HiBiT Extra Cellular Detection System on the CyBio FeliX

Automated 96-well protein quantification

Rapid, 96-well protein quantification has been optimized on the CyBio FeliX, facilitating high-throughput screening and identification of optimal solutions. This protein quantification is achieved using the Nano-Glo® HiBiT Extracellular Detection System from Promega. This assay permits precise quantification of extracellular and secreted HiBiT-tagged proteins.


Benefits of  automating 

  • Easy setup

  • Process in minutes   

  • Automated standard curve generation 

  • Parallel 96 transfers 

  • Scalable & reproducible

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Protein quantification on the CyBio FeliX

For high-throughput quantification, proteins of interest can be modified with the HiBiT tagging technology. HiBiT is a small, 11-amino-acid, epitope tag capable of producing a bioluminescent signal when bound to its complementation partner, LgBiT. 

Tagged proteins can then be quickly setup for quantification on the CyBio FeliX via the Nano-Glo® HiBiT Extracellular Detection System. For maximum throughput, cultures can be grown directly in 96-well plates and sampled directly with the 96 tip-tray format available with the CyBio FeliX. 

Standard curve generation

A standard curve by which the protein concentration of samples can be assessed is first generated. Reagents and the control protein are loaded onto the FeliX for the serial dilution.

Using a single tip, reagents are transferred to the plate, before a serial dilution is then performed. 

Tell the CyBio FeliX how many dilution steps you want for automatic setup!

Reagent plate setup

The HiBit reagent buffers are then aliquoted by the CyBio FeliX into a 96-well format for parallel addition.

Quantifying more than one 96 well plate?

Simply load more plates onto the deck and quickly fill them all with 96 tip-tray instead of an 8-channel column. 


Sample quantification

Once the plate is pre-filled with HiBit Reagent, all that is remaining is to add the sample to be quantified. This can be purified protein or often cell culture supernatant.

With the 96 tip-tray, all your samples are transferred in parallel to the assay plate. This ensures any variation due to sample-reagent incubation time is minimized. 

Four separate 96 well plates can be processed without user intervention!

Hear from one of our customers EdenBio

The CyBio FeliX in combination with the Head R 96/60 µL can easily handle protein engineering workflows which require large sample numbers are generated. From setup to quantification, the whole process can be done in a matter of minutes. 

One of our customers EdenBio, a biotechnology company leveraging machine learning and automation, has benefited from the CyBio FeliX to rapidly scale their throughput to meet customer demands. You can watch their automation journey here, and read our application note below. 

This benchtop package can automate the entire strain engineering workflow in a small footprint, ideal for busy labs with little space. This includes applications such as gene assembly, transformation, cell & microbial culture, protein quantification and targeted protein purification. 

Learn more about our solutions

CyBio FeliX Brochure


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Automated 96-well protein quantification with the Nano-Glo® HiBiT Extra Cellular Detection System on the CyBio FeliX.

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