Biometra Rotaphor

Exceptional resolution and reliable nucleic acid analysis
Intuitive PC software
Suitable for a variety of methods: CHEF, ROFE, PAGE

Product Details

Specially designed for the separation of very large DNA molecules
The Biometra Rotaphor is a rotating field electrophoresis systemspecifically designed for the high resolution separation of large DNA molecules up to 8 MB in agarose gels under controlled temperatur conditions. The unique combination of a patented rotor technology, an integrated temperature control system and a clearly structured user interface meets all requirements of routine handling and make the analysis of large nucleic acids simple and uncomplicated.

Excellent separation quality for large DNA fragments up to 8 MB
Increased resolution through patented rotor technology
High-precision temperature control system guarantees excellent temperature homogeneity, ensuring the accuracy of the results

Efficiency and reliability through innovative rotor technology
Pulsed field electrophoresis with the Rotaphor system allows the separation of high molecular weight nucleic acids. Thanks to the patented electrode rotor, the electrical field can be applied in virtually any angle.
Freely editable program functions (puls interval, angle and field strength) in combination with the freely rotatable electrodes offer a maximum of flexibility with the highest angular accuracy.

Rotating electrodes allow the free setting of homogeneous field vectors
Nonlinear switching time ramping
Suitable for versatile separation techniques: CHEF, ROFE

Rotaphor operating software - the basis for your success
The Rotaphor software convinces with its comfortable operation, real-time control of relevant test parameters and 17 preinstalled application programs for the analysis of DNA fragments of various size ranges. Benefit from our expertise and save valuable time in optimizing your own experiments.

17 preinstalled programs, optimized for different size ranges
Easy customization of complex protocols

Technical Data

Rothaphor
Dimensions (W x D x H)	35 x 47 x 25 cm
Lid	Freely rotating electrode rotor, acryl glass (automatic shut off when lid is lifted)
Weight	12 kg
Electrodes	14 (on the rotor; 2 main-, 12 secondary electrodes), platinum
Buffer volume required	2.4 l
Temperature monitoring	Precise control of the buffer temperature between 5 ° C and 22 ° C by integrated cooling coil and a temperature sensor in base of chamber; Note: A connection to an external cooling unite is needed
Operating temperature	10 - 13 °C recommended
Allowable voltage gradients	1–8,5 V/cm (30-225 V in 1 V increments)
Pre installed programs	17 optimized programs for separation of samples from 100 kb up to 6 Mb
Alarm function	Yes
Automatic restart after power failure	Yes
Pump for buffer recirculation	Yes
Controller	PC
Display	PC Monitor
Fuses	200 mA Medium-Blow

Power Module
Dimensions (W x D x H)	5.2 x 22.2 x 20.2 cm
Weight	1.7 kg
Max. Voltage	300 V
Max. Current	0.45 A
Program Functions
Pulses	Defined by voltage, angle, and duration
Extra Mode	Current can be applied also during rotor movements (especially for small fragments)
Program length	1 - 500 hours
Switch puls length	1 - 10.000 s (linear or logarithmic)
Switch angle	0°, 90°, 91°-160° (linear or logarithmic)
Switch voltage	30 - 225 V (linear or logarithmic)

Accessories Included
Casting stand	20 x 20 cm
Comb	18-well comb, 2.0 mm
Moulding form	for 20 agarose embedded samples
Gel scoop	14 x 15 cm, UV-transparent
Computer	with pre-installed Windows™ operating system, Rotorgene software and controller card
22'' TFT Monitor
Keyboard
Mouse

Cooling Module (optional)
Working temperature range	-10 °C bis +80 °C
Fixed temperatures	5 (free selection)
Temperature accuracy	± 0,1 K
Display	Multifunction, digital
Heating capacity	2.000 W
Cooling capacity	250 W of input power at 20 °C
Pump flow rate	7,5 I/min or 15/min
Max. pressure	300 mbar
Bath volume (internal)	2.8 I
Dimensions (W x L x H)	23.2 x 4.7 x 62.0 cm
Weight	30 kg

Order Information

Order number	Item
846-021-101	Rotaphor 8 System, 230 V, PC with Windows operating system (German)*
846-021-201	Rotaphor 8 System, 110 V/230 V, PC with Windows operating system (English)*
846-021-202	Rotaphor 8 System, 230 V, China-RoHS-conform PC with Windows operating system (English)*
	* The Rotaphor System includes: Electrophoresis chamber with electrode rotor and internal buffer circulation, power supply, gel tray, comb (18 teeth), frame for gel casting, sample mould, connection cables, computer with interface card and pre-installed Rotaphor software, 22" TFT monitor, gel scoop, manual
846-043-500	Refrigerating circulator Accel 250 (230 V), operating temperature range - 10 °C to + 80 °C
846-043-590	Refrigerating circulator Accel 250 (115 V), operating temperature range - 10 °C to + 80 °C

Order number	Accessories
	Combs for 20 cm x 20 cm gels
846-021-011	18 wells
846-021-004	25 wells
846-021-005	40 wells
846-021-006	50 wells
846-021-007	18 wells (for liquid samples)
	Combs for 18 cm x 13 cm gels
846-021-008	5 wells
846-021-009	12 wells
846-021-010	20 wells
846-021-003	Sample casting form (for 20 embedded samples, 14 mm x 10 mm x 1 mm each)
846-021-001	Preparative gel casting kit, 13 cm x 18 cm gel casting tray, comb 5 wells (3 marker slots, 2 preperative slots), comb 12 wells
846-021-002	Gel tray (20 cm x 20 cm gels) and casting frame

Downloads

Product Brochure Biometra Rotaphor System 8 (Separation of Large DNA Molecules)

Open PDF