Biometra Rotaphor 8 System

  • Separation of large DNA molecules (up to 8 Mb = 8,000 kb)
  • Patented electrode rotor allows free electrical field angle
  • Exceptional resolution
  • Optimized protocols for different size ranges

Product Details

Pulsed Field Gel Electrophoresis (PFGE) System for the Separation of Large DNA Molecules – Rotating field electrophoresis (ROFE)
Pulsed field electrophoresis with the Rotaphor system allows the separation of DNA beyond the 50 kb limit of conventional agarose electrophoresis. Thanks to the patented electrode rotor, the electrical field can be applied in virtually any angle. To achieve a very homogeneous field, the two main rotor electrodes are flanked by 2 sets of secondary electrodes each.

Flexibility
Due to the unique design of the Rotaphor system, all common techniques for separation of large DNA molecules can be applied. This includes methods like CHEF, FIGE, PAGE and naturally ROFE (Rotating Field Electrophoresis).

Buffer management
Since PFGE gels typically run for many hours (up to several days), the electrophoresis buffer has to be cooled. The Rotaphor electrophoresis chamber comes with a built-in buffer circulation pump which is connected to an external cooling thermostat. During the run the buffer temperature in the electrophoresis chamber is constantly monitored and precisely controlled by the Rotaphor software.

Easy control
The Rotaphor system includes a PC that controls the electrode rotor and the power supply over the Rotaphor interface card. The Rotaphor 8 software provides 17 pre-set programs for separation of different size ranges. Starting with the pre-set parameters, new applications are quickly optimised. For each pre-set program the software shows a real gel image. Once a custom program has been optimised, a referring gel picture can be uploaded into the software. Thus, the Rotaphor software over time will not only be a control tool, but also a library of successful experiments linked to the underlying separation parameters. By linking programs to combined lists also complex sequences can be programmed.

Order Information

Order numberItem
846-021-101Rotaphor 8 System, 230 V, PC with Windows operating system (German)*
846-021-201Rotaphor 8 System, 110 V/230 V, PC with Windows operating system (English)*
846-021-202Rotaphor 8 System, 230 V, China-RoHS-conform PC with Windows operating system (English)*
 * The Rotaphor System includes: Electrophoresis chamber with electrode rotor and internal buffer circulation, power supply, gel tray, comb (18 teeth), frame for gel casting, sample mould, connection cables, computer with interface card and pre-installed Rotaphor software, 22" TFT monitor, gel scoop, manual
846-043-500Refrigerating circulator Accel 250 (230 V), operating temperature range - 10 °C to + 80 °C
846-043-590Refrigerating circulator Accel 250 (115 V), operating temperature range - 10 °C to + 80 °C
Order numberAccessories
 Combs for 20 cm x 20 cm gels
846-021-01118 wells
846-021-00425 wells
846-021-00540 wells
846-021-00650 wells
846-021-00718 wells (for liquid samples)
 Combs for 18 cm x 13 cm gels
846-021-0085 wells
846-021-00912 wells
846-021-01020 wells
846-021-003Sample casting form (for 20 embedded samples,
14 mm x 10 mm x 1 mm each)
846-021-001Preparative gel casting kit, 13 cm x 18 cm gel casting tray, comb 5 wells (3 marker slots, 2 preperative slots), comb 12 wells
846-021-002Gel tray (20 cm x 20 cm gels) and casting frame

Downloads

Product Brochure Biometra Rotaphor System 8 (Separation of Large DNA Molecules)

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