Biometra Rotaphor

  • Exceptional resolution and reliable nucleic acid analysis
  • Intuitive PC software
  • Suitable for a variety of methods: CHEF, ROFE, PAGE

Product Details

Specially designed for the separation of very large DNA molecules
The Biometra Rotaphor is a rotating field electrophoresis systemspecifically designed for the high resolution separation of large DNA molecules up to 8 MB in agarose gels under controlled temperatur conditions. The unique combination of a patented rotor technology, an integrated temperature control system and a clearly structured user interface meets all requirements of routine handling and make the analysis of large nucleic acids simple and uncomplicated.

  • Excellent separation quality for large DNA fragments up to 8 MB
  • Increased resolution through patented rotor technology
  • High-precision temperature control system guarantees excellent temperature homogeneity, ensuring the accuracy of the results

Efficiency and reliability through innovative rotor technology
Pulsed field electrophoresis with the Rotaphor system allows the separation of  high molecular weight nucleic acids. Thanks to the patented electrode rotor, the electrical field can be applied in virtually any angle.
Freely editable program functions (puls interval, angle and field strength) in combination with the freely rotatable electrodes offer a maximum of flexibility with the highest angular accuracy.

  • Rotating electrodes allow the free setting of homogeneous field vectors
  • Nonlinear switching time ramping
  • Suitable for versatile separation techniques: CHEF, ROFE

Rotaphor operating software - the basis for your success
The Rotaphor software convinces with its comfortable operation, real-time control of relevant test parameters and 17 preinstalled application programs for the analysis of DNA fragments of various size ranges. Benefit from our expertise and save valuable time in optimizing your own experiments.

  • 17 preinstalled programs, optimized for different size ranges
  • Easy customization of complex protocols  

Technical Data

Rothaphor
Dimensions (W x D x H)35 x 47 x 25 cm
LidFreely rotating electrode rotor, acryl glass (automatic shut off when lid is lifted)
Weight12 kg
Electrodes14 (on the rotor; 2 main-, 12 secondary electrodes), platinum
Buffer volume required2.4 l
Temperature monitoringPrecise control of the buffer temperature between 5 ° C and 22 ° C by integrated cooling coil and a temperature sensor in base of chamber;
Note: A connection to an external cooling unite is needed
Operating temperature10 - 13 °C recommended
Allowable voltage gradients1–8,5 V/cm (30-225 V in 1 V increments)
Pre installed programs17 optimized programs for separation of samples from 100 kb up to 6 Mb
Alarm functionYes
Automatic restart after power failureYes
Pump for buffer recirculationYes
ControllerPC
DisplayPC Monitor
Fuses200 mA Medium-Blow
Power Module
Dimensions (W x D x H)5.2 x 22.2 x 20.2 cm
Weight1.7 kg
Max. Voltage300 V
Max. Current0.45 A
Program Functions 
PulsesDefined by voltage, angle, and duration
Extra ModeCurrent can be applied also during rotor movements (especially for small fragments)
Program length1 - 500 hours
Switch puls length1 - 10.000 s (linear or logarithmic)
Switch angle0°, 90°, 91°-160° (linear or logarithmic)
Switch voltage30 - 225 V  (linear or logarithmic)
Accessories Included
Casting stand20 x 20 cm
Comb18-well comb, 2.0 mm
Moulding formfor 20 agarose embedded samples
Gel scoop14 x 15 cm, UV-transparent
Computerwith pre-installed Windows™ operating system, Rotorgene software and controller card
22'' TFT Monitor 
Keyboard 
Mouse 
Cooling Module (optional)
Working temperature range -10 °C bis +80 °C
Fixed temperatures 5 (free selection)
Temperature accuracy ± 0,1 K
DisplayMultifunction, digital
Heating capacity2.000 W
Cooling capacity250 W of input power at 20 °C
Pump flow rate7,5 I/min or 15/min
Max. pressure300 mbar
Bath volume (internal)2.8 I
Dimensions (W x L x H)23.2 x 4.7 x 62.0 cm
Weight30 kg

Order Information

Order numberItem
846-021-101Rotaphor 8 System, 230 V, PC with Windows operating system (German)*
846-021-201Rotaphor 8 System, 110 V/230 V, PC with Windows operating system (English)*
846-021-202Rotaphor 8 System, 230 V, China-RoHS-conform PC with Windows operating system (English)*
 * The Rotaphor System includes: Electrophoresis chamber with electrode rotor and internal buffer circulation, power supply, gel tray, comb (18 teeth), frame for gel casting, sample mould, connection cables, computer with interface card and pre-installed Rotaphor software, 22" TFT monitor, gel scoop, manual
846-043-500Refrigerating circulator Accel 250 (230 V), operating temperature range - 10 °C to + 80 °C
846-043-590Refrigerating circulator Accel 250 (115 V), operating temperature range - 10 °C to + 80 °C
Order numberAccessories
 Combs for 20 cm x 20 cm gels
846-021-01118 wells
846-021-00425 wells
846-021-00540 wells
846-021-00650 wells
846-021-00718 wells (for liquid samples)
 Combs for 18 cm x 13 cm gels
846-021-0085 wells
846-021-00912 wells
846-021-01020 wells
846-021-003Sample casting form (for 20 embedded samples,
14 mm x 10 mm x 1 mm each)
846-021-001Preparative gel casting kit, 13 cm x 18 cm gel casting tray, comb 5 wells (3 marker slots, 2 preperative slots), comb 12 wells
846-021-002Gel tray (20 cm x 20 cm gels) and casting frame

Downloads

Product Brochure Biometra Rotaphor System 8 (Separation of Large DNA Molecules)

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