innuSCRIPT One Step RT-PCR Probe Kit

2x master mix uniting reverse transcription with subsequent qPCR in a single reaction vessel

Product Details

  • Contains thermostable reverse transcriptase (50° C to 55° C) and an effective RNase inhibitor
  • Reverse transcription followed by real-time PCR in a single step
  • Highly sensitive
  • Universally applicable: standard and fast cyclers, GC- and AT-rich templates
  • Simple handling and superior reproducibility
  • Fast amplification rate for Ct values that can be detected early

The innuSCRIPT One Step RT-PCR Probe Kit is a ready-to-use, 2x master mix uniting reverse transcription with subsequent qPCR in a single reaction vessel. Positive test results are already available for the reaction mix on a variety of commercially available real-time thermal cyclers. The innuSCRIPT One Step RT-PCR Probe Kit is based on cDNA synthesis and subsequent real-time PCR using specific probe-detection technologies. The kit composition was validated in such a way that each RNA template can be quantified, including mRNA, total RNA and viral sequences. Detection is also highly efficient for targets for which the number of copies is extremely small. Adding RNA template and primer to the reaction are the only preparation steps involved. The final step is to add PCR-grade water to achieve the final volume.

Specifications

Concentration

2x master mix

PS-Mg2+ solution

The concentration of MgCl2 in the master mix has been optimized, eliminating the need for adding more MgCl2.

Order Information

Order numberQuantity
845-RT-70001001 mL for 100 reactions of 20 μL each
845-RT-70002002 mL for 200 reactions of 20 μL each

Downloads

Brochure Product Guide Reagents Consumables

Open PDF

Manual innuSCRIPT One Step RT PCR Probe Kit

Open PDF

Contact us We are happy to help you and will contact you as quickly as possible.