RoboGene HCV RNA Quantification Kit 3.0

CE-IvD marked real-time PCR based detection system for quantification of Hepatitis C Virus (HCV) RNA

Product Details

  • Simplified kit format extensively validated on qTOWER³ as well as several other real-time PCR platforms
  • Precise acquisition of clinical data due to four included lyophilized quantitation standards
  • Improved sensitivity: down to 13.6 IU/mL (manual extraction) and 11.7 IU/mL (automated extraction)
  • Reduced qPCR run times down to < 2 h

The RoboGene HCV RNA Quantification Kit 3.0 is validated for quantification of Hepatitis C Virus RNA in human plasma or serum samples using One-Step Real-Time PCR. The universal kit setup allows flexible and reliable application to most major Real-Time PCR platforms currently available on the market.

The Internal Control is provided as an IC Spiking Tube for convenient handling with manual as well as automated extraction methods. Due to probes labeled with different fluorescent reporter dyes, amplification of HCV RNA and IC in samples and standards are measured independently at different wavelengths (HCV in FAM Channel, IC in VIC or Cy5 channel). Benefit from superior sensitivity and specificity and rely on defined lyophilized standards covering concentrations from 2.6 to 7.6 log IU/mL for precise data acquisition. Three kit sizes, all including 4x4 standards, are available in order to provide the perfect solutions from low to high throughput laboratories.

Specifications

Starting material

RNA from human plasma and serum

Target

5´untranslated region

Detection principle

Quantitative qPCR (TaqMan®)

Linear range

50 IU/mL - 4x1010 IU/mL

Validated qPCR platforms

qTOWER³ (AJ), CFX 96 (Biorad), LightCycler® 480 II (Roche), Rotor-Gene® 3000/6000/Q (Corbett Research/ Qiagen), 7500 Fast (ABI)  

Detection time

< 120 min

Sensitivity

13.6 IU/mL (manual extraction)

11.7 IU/mL (automated extraction) 

 

 

Order Information

Order numberQuantity
847-020761003232 reactions
847-020761009696 reactions
847-0207610192192 reactions

 

Manufacturer:

AJ Roboscreen GmbH
Hohmannstrasse 7
04129 Leipzig
Germany
Phone: +49 341 989734 0
Fax: +49 341 989734 199

Downloads

EC Certificate RoboGene HCV RNA Quantification Kit 3.0

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IFU_RoboGene HCV RNA Quant Kit 3.0

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Br_RoboGene Family_en

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