SARS-CoV-2 (Coronavirus) Detection Workflow for research use only

Fri 11 Sep, 2020

Overview of the SARS-CoV-2 (Coronavirus) Detection Process

In December 2019 the novel SARS-CoV-2 (formerly named 2019-nCoV or commonly named coronavirus) was identified in Wuhan, the capital of China’s province Hubei. Based on its rapid spreading with more than 56,000,000 confirmed cases by the mid of November 2020 the World Health Organization declared the outbreak a public health emergency of international concern.

Important facts:

  • SARS-CoV-2: (+) ss-RNA virus
  • Related to SARS-CoV and MERS-CoV
  • Detection based on basis of PCR

Analytik Jena offers a broad product portfolio in the field of nucleic acid extraction and PCR or real-time PCR with both extraction kits and the necessary equipment. For research purposes for the detection of SARS-CoV-2 the following workflow is suggested.

1. Sample Collection

In principle, respiratory material such as nasopharyngeal and oropharyngeal swabs are suitable for the detection of respiratory pathogens. For more detailed information, recommendations of the WHO or other institutions should be observed.
Serum can also be used for serological tests, acute and convalescence samples.

2. Nucleic Acid Extraction

Analytik Jena offers a choice of extraction platforms and related extraction kits for extraction of viral RNA from swabs, cell free body fluids (e.g. serum, plasma, cerebrospinal fluid) and more. The product line innuPREP AniPath allows the simultanous extraction of both bacterial and viral DNA and/or RNA from respective starting materials. A detailed overview is shown in the table below.

HandlingSample throughputExtraction platformExtraction kit
NameKey features
ManualLow-Up to 12 samples / 25 mininnuPREP Virus TS RNA Kit



MiddleInnuPure C16 touchUp to 16 samples / 31 - 84 min
Extraction platform only
innuPREP AniPath DNA/RNA Kit - IPC16, non-filled
innuPREP Virus DNA/RNA Kit - IPC16, non-filled
HighCyBio FeliXUp to 96 samples / 62 - 73 min
Extraction platform + liquid handling
(e.g. preparation of PCR)
innuPREP AniPath DNA/RNA Kit – FX*
innuPREP Virus TS RNA Kit 2.0 - FX
HighKingFisher FlexUp to 96 samples / 60 min
Extraction platform only
innuPREP AniPath DNA/RNA Kit - KFFLX

* Release planned for December 2020

3. Amplification and Detection

Molecular assays to detect SARS-CoV-2 have been developed and are accessible through the homepage of the WHO. Furthermore, several commercial kits based on Real-time PCR are available such as:

  • Altona Diagnostics: RealStar ® SARS-CoV-2 RT-PCR Kit 1.0***
  • BGI Genomics: Real-Time Fluorescent RT-PCR Kit for Detecting SARS-2019-nCoV***
  • EuroImmun: EuroRealTime SARS-CoV-2****
  • PerkinElmer: SARS-CoV-2 RT-qPCR Reagent kit****
  • Fosun Pharma: Fosun COVID-19 RT-PCR Detection Kit***
  • Ingenetix: ViroReal ® SARS-CoV-2 & SARS****
  • Procomcure Biotech: PhoenixDx® SARS-CoV-2 IVD****
  • Primerdesign: 2019-nCoV genesig® Advanced kit***
  • R-Biopharm: RIDA®GENE SARS-CoV-2 RUO***
  • Seegene: AllplexTM 2019-nCoV Assay***
  • Siemens Healthineers: Fast Track Diagnostics SARS-CoV-2 Assay***
  • Shimadzu: 2019 Novel Coronavirus Detection Kit***
  • TIB MOLBIOL: LightMix® Modular SARS-CoV (COVID19) E-gene***
  • TIB MOLBIOL: LightMix® Modular SARS-CoV (COVID19) RdRP***
  • TIB MOLBIOL: LightMix® Modular EAV RNA Extraction Control***

*** Confirmed compatibility with qTOWER³
****  Validated on qTOWERG by assay manufacturer

The PCR set up can be pipetted by a liquid handling platform like CyBio FeliX . Using PCR workstations or cabinets increases maximum safety and minimizes contamination risks. Single components such as qPCR Master Mixes or others are available via Analytik Jena as well. In general all Real-time PCR-based assays can be established on the qTOWER3 Real-time thermal cycler family provided by Analytik Jena or similar.
Information on further commercial qPCR assays for SARS-CoV-2 detection with confirmed compatibility on qTOWER3 devices is available on request.

Please note that all Analytik Jena GmbH products listed here are exclusively intended for research use only unless explicitly marked as CE-IVD.


Flyer Workflow detection of SARS-CoV-2 (English)

Open PDF

Implementation of SARS-CoV-2 (Coronavirus) detection assays from TIB MOLBIOL on qTOWER³ for research use

Open PDF

Web Seminar Biofoundries and SARS-CoV-2 diagnostics

Learn more: Web Seminar Biofoundries and SARS-CoV-2 Diagnostics


Comments (19)

  • Abhimanyu Pandey

    thank you
    • Analytik Jena AG

      You are welcome!
  • Mustakim,MD,Clin.Path

    Thank you,I am User Analytic Jenna PCR labkesda Soppeng From South Celebes Indonesia.
  • Florian Werner

    We have also validated our Kylt SARS-CoV-2 Kits on the qTower 384. Very satisfied with the performance and results. (
  • Bhuneshwar Behra

    Thank you
  • Sunil Prasad Lekhak

    Dear Sir/Madam

    We are trading company in Nepal, specialized in Molecular Diagnostic lab.
    Currently we are looking for Fully automated Nucleic acid extraction system and liquid handling system.
    I am very much interested in your CyBio Felix system.

    Could you please send me technical specifications and Distributor Price for Nepal.

    Looking forward to your reply.

    Sunil Prasad Lekhak

    Life Sciences Solutions Pvt. Ltd.
    • Analytik Jena AG

      Thank you for your inquiry. We have forwarded your request to our sales team which will contact you as soon as possible.
  • Jazz

    I Am looking into doing research for covid kit for salmon , poultry etc sample, can I ask if your kit suitable for animal sample ? What is the price to run a covid test? If the test including extraction and regained as well? Can you quote both ? What is the lead time and shelf life of this ?
    • Analytik Jena AG

      Thank you for your inquiry. Our sales team will contact you shortly to answer your questions.
  • Mario Schwarzkopf

    Hallo, ich Interessiere mich auch für ein Kit mit dem schnellstmöglich das Virus nachgewiese, bzw. ausgeschlossen werden kann. Ich bräuchte alle Gerätschaften, die Notwendig wären um dies für eine Mobile Prüfung zu nutzen. Würde mich über nähere Informationen und Preise freuen.

    Beste Grüße
    • Analytik Jena AG

      Hallo Herr Schwarzkopf, wir haben Ihre Anfrage an unser Sales-Team weitergeleitet, welches die genauen Einsatzzwecke und Gegenheiten mit Ihnen abklären wird.
  • Sara Sánchez

    Cordial saludo,

    Quisiera saber si hay un proceso de validación con el kit comercial Molecutech Real Time COVID-19 el cual nos puedan compartir. O si hay opción de hacer una capacitación virtual para la interpretación de los resultados de las corridas realizadas con el qTower 3 y el Kit de Molecutech.
    • Analytik Jena AG

      Dear Mrs. Sánchez, right now the kit isn't validated on qTOWER3 yet. Please contact our application specialists at I'm sure that you will find an appropriate solution for your setup together.
  • Mudhafar

    Are SARS-CoV-2 (Coronavirus) Kits from Analyticgena Working with Applied Biosystem 7500 fast Device?
    • Analytik Jena AG

      Analytik Jena offers various nucleic acid extraction kits which can be used for the purification of viral RNA such as the SARS-CoV-2. For the subsequent detection via RT real time PCR on the one hand the PCR assay amplifying SARS-CoV-2-specific targets via distinct fluorescent dyes or probes is required. Such SARS-CoV-2 assays are not part of Analytik Jena's product portfolio. On the other hand a real time cycler is needed (which is offered by AJ). The PCR Assay and real time cyclers have to be compatible. The sample preparation via nucleic acid extraction does not affect the real time cycler so it is no problem to do nucleic acid extraction with kits by Analytik Jena and PCR with any cyler such as the Applied Biosystem 7500 fast Device .
  • Michael

    What cell free body fluid is the “liquor” part? That is from your web site above:

    2. Nucleic Acid Extraction
    Analytik Jena offers a choice of extraction platforms and related extraction kits for extraction of viral RNA from swabs, cell free body fluids (e.g. serum, plasma, liquor) and more.
    • Analytik Jena AG

      The aforementioned body fluid is cerebrospinal fluid. Thank you for the hint! We have corrected the text passage.
  • Mary

    Dear Sir/ Madam,

    We are using the KH medical etraction and detection kits for COVID-19 Virus. We already have the qTower2.0 at our institute but it seems not to give good amplification/ detection curves in the same. should we upgrade it or what could be the problem?
    • Analytik Jena AG

      Dear Mary, thank you for reaching out to us. The qTOWER2 should be able to help with your COVID-19 detection. Maybe a software update is all that is necessary. Our Life Science support staff has been contacted to assist you in solving this problem.

Write new comment


Contact us