PME free-circulating DNA Kit-IPC16

Automized extraction of free-circulating DNA

Product Details

  • Processing of starting sample volumes up to 10 ml
  • Novel, patent pending technology: Polymer Mediated Enrichment (PME)
  • Enrichment & extraction in approx. 76 minutes from 1 ml or approx. 1,5 h from 5 ml of serum or plasma and up to 10 ml from urine
  • For usage of InnuPure C16 and up to 16 samples in parallel

Circulating cell-free DNA is a very interesting diagnostic target, but the amount of freecirculating DNA is usually very low and varies among different individuals. Further, these nucleic acids are present as short fragments, typically smaller than 1000 nt, making the efficient extraction process challenging. Because of the high sample volumes the protocols of commercially available kits are very labor-intensive as well as time consuming and need a lot of reagents.
The PME free-circulating DNA Kit-IPC16 is based on a new, patent-pending technology called PMEPolymer Mediated Enrichment. As first step cell-free DNA in the entire sample is captured by a polymer. Afterwards this complex is collected as a pellet by centrifugation. Subsequently the captured nucleic acid is dissolved in a special buffer thus reducing the sample volume in the following extraction significantly. Subsequently the extraction of free-circulating DNA from up to 16 samples in parallel takes place within InnuPure C16

Specifications

Extraction Time

Enrichment (external):

  • From 1 ml starting sample: approx. 10 minutes
  • From 5 ml and 10 ml starting sample: approx 30 minutes
  • InnuPure C16: approx. 66 minutes (1 ml) - 70 minutes (5 – 10 ml)

Starting material

  • Serum, plasma and urine
  • Cell culture supernatants or mediums
  • Other cell-free body fluids
  • From up to 5 ml (serum and plasma) or 10 ml (urine)

Order Information

Order numberQuantity
845-IPS-611601616 reactions
845-IPS-611609696 reactions
845-IPP-611601616 reactions
845-IPP-611609696 reactions
IPS = Kit contains prefilled reagent strips for processing individual samples
IPP = Kit contains prefilled reagent plates for running 8 samples in parallel
Note: Prefilled reagent strips and reagent plates can be used in parallel in the InnuPure C16

Downloads

Product Flyer PME

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Manual PME free circulating DNA Kit IPC16

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MSDS PME free circulating DNA Kit IPC16

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Other Devices

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