innuSPEED Tissue RNA Kit

Isolation of total cellular RNA from a variety of tissues

Product Details

  • Kit contains Lysis Tubes with beads for homogenization
  • Optimized for using the SpeedMill
  • May also be used with other homogenizers
  • Improved extraction without the use of toxic β-mercaptoethanol

The innuSPEED Tissue RNA Kit allows users to isolate total RNA from a variety of tissue samples quickly and efficiently through the use of a homogenizer. A SpeedMill (or other homogenizer) is used as an effective means of digesting the starting material within the Lysis Tubes contained in the kit. Lysis is performed without the use of highly toxic β-mercaptoethanol, after which the genomic DNA is removed through an initial Spin Filter column. The RNA is then bound to a second Spin Filter membrane, washed and finally eluted in 30 - 80 µl of RNase-free water.

Specifications

Starting material

  • Tissue samples of up to 20 mg
  • Bioptates

Average yield

  • Depends on the type and quantity of the starting material
  • Up to 100 µg

Extraction time

  • Homogenization and lysis: approx. 20 minutes
  • Purification: approx. 11 minutes

Binding capacity

Column binding capacity: > 100 µg RNA

Average purity

1,7 – 2,0

Order Information

Order numberQuantity
845-KS-254001010 reactions
845-KS-254005050 reactions

Downloads

Other Devices

SpeedMill PLUS

The SpeedMill PLUS is a highly efficient homogenization system for various starting materials used for the subsequent isolation and purification of DNA, RNA, or proteins.

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