innuPREP RNA Kit–IPC16

Easy, automated extraction of total RNA

Product Details

  • Optimized for isolating total RNA from a wide variety of tissue samples and cells
  • No need for highly toxic ß-mercaptoethanol
  • Spin Filter column for selective DNA removal reduces time required (no DNase I digestion)
  • Greatly reduced contamination risk
  • Kit contains all consumables and prefilled reagents
  • Based on magnetic particle separation in the InnuPure C16 touch

The innuPREP RNA Kit-IPC16, which has been optimized for the InnuPure C16 touch, makes it possible to automate RNA extraction from a wide variety of tissue samples and cells. Lysis is especially efficient and requires no more than 20 to 30 minutes to complete (depending on the starting material. Genomic DNA is then selectively removed via centrifugation with Spin Filter columns, a step that eliminates the need for time-consuming enzymatic DNA digestion. Subsequent steps, which include binding and washing the total RNA, as well as the final elution step, proceed in the InnuPure C16 touch and are fully automated. The extraction process is based on separation by magnetic particles, which bind RNA due to the buffer conditions adjusted. Prefilled Reagent Strips or Reagent Plates, prevent any potential contamination. The magnetic particles and extraction protocols have been specially adapted for isolating RNA and, as such, guarantee highly pure RNA and excellent yields.

Specifications

Starting Material

  • Eukaryotic cells (max. 5 x 106)
  • Various tissue samples (max. 20 mg)
  • Tissue samples with a high RNA content (e.g. spleen samples, pan-creatic samples, lymph nodes, max. 5 mg)

Extraction Time

  • Lysis: Cells approx. 10 to 20 minutes
  • Tissue depending on type of tissue and homogenization
  • InnuPure C16 touch protocol: approx. 54 minutes

Average Yield

  • Depending on type and amount of starting material used
  • Eukaryotic cells (e.g. NIH 3T3 or HEK 293) up to 20 μg
  • Tissue samples (e.g. mouse spleen) up to 50 μg

Average Purity

1,8 – 2,1

Order Information

Order numberQuantity
845-IPS-411601616 reactions
845-IPS-411609696 reactions
845-IPP-411601616 reactions
845-IPP-411609696 reactions
845-IPP-4116480480 reactions
 
IPS = Kit contains prefilled reagent strips for processing individual samples
IPP = Kit contains prefilled reagent plates for running 8 samples in parallel
Note: Prefilled reagent strips and reagent plates can be used in parallel in the InnuPure C16 touch
 

Downloads

Brochure Product Guide Extraction

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Manual innuPREP RNA Kit IPC16

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MSDS innuPREP RNA Kit IPC16

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Other Devices

InnuPure C16 touch

InnuPure C16 touch is the new standard in automated extraction. It offers efficient fully automatic nucleic acid extraction.

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