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Soluciones de Analytik Jena para PCR en tiempo real Explore aplicaciones avanzadas de qPCR multiplex

Content of the web seminar

Duration: ~60 min
Language: Spanish
Speakers:
​​Marco Noronha, Application Specialist, Analytik Jena GmbH+Co.KG

En este seminario se presentarán los fundamentos de la PCR en tiempo real y su papel en diversas aplicaciones de laboratorio, con enfoque en la transición hacia enfoques multiplex. Se discutirán las principales diferencias entre ensayos singleplex y multiplex, destacando ventajas, limitaciones y estrategias prácticas de optimización.

También se abordará la preparación automatizada de reacciones de qPCR utilizando el sistema CyBio FeliX, enfatizando mejoras en reproducibilidad, estandarización y eficiencia, junto con una visión general de los pasos involucrados en el flujo de trabajo automatizado.

Finalmente, se explorará el uso del qTOWERiris como solución para qPCR multiplex, incluyendo conceptos clave como compensación de color, configuración de experimentos y ejemplos de aplicación práctica, demostrando cómo simplificar análisis complejos con mayor confiabilidad y desempeño.

Key learning objectives:

  • Comprender los principios de la qPCR y las diferencias entre los enfoques singleplex y multiplex.
  • Identificar los desafíos de la qPCR multiplex y aplicar estrategias de optimización para obtener resultados confiables.
  • Evaluar los beneficios de la automatización con CyBio FeliX y el uso de qTOWER iris para simplificar experimentos multiplex.

Target groups:

  • Laboratorios de investigación en biología molecular y genética
  • Laboratorios de diagnóstico clínico y molecular
  • Industrias farmacéutica y biotecnológica

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