Highest precision with 1.5-fold discrimination for qPCR quantification applications Application Note · qTOWER3

Introduction

A valid real-time PCR assay is characterized by four major properties: PCR efficiency, reproducibility, specificity and sensitivity. Several factors have an influence on these critical points; beside primer design, plastic ware and master mix, of course the performance of the real-time PCR cycler itself affects the results. For this reason, it is mandatory to guarantee best properties of the real-time PCR cycler to rely on the outcome of the applied real-time PCR.

One of the most challenging requirements is to discriminate between small dilution steps, even down to 1:1.5, which proves the dependability and accuracy of the performance of the real-time PCR cycler and therefore of all data created with the device.