Biometra Tank-Blotting For gentle blotting of large and temperature-sensitive proteins

  • Best suited for large proteins and native enzymes
  • Fast, efficient and reproducible transfer of proteins
  • Effective cooling thanks to built-in cooling
  • Simultaneous blotting of up to four gels

Product Details

Tank Blot Apparatus and Blotting Modules For Gentle Blotting of Large and Temperature-Sensitive Proteins

Best suited for large proteins and native enzymes
During tank blotting proteins are transferred in a vertical buffer tank between electrodes arranged on the sidewalls onto membranes. Tank blotting is recommended particularly for blotting of large molecules (> 100 resp. 200 kDa, depending on the proteins characteristics) or of proteins which are difficult to transfer with other blotting techniques. It is the method of choice also for native and temperature-sensitive proteins. Transfer can be done fast or overnight. Thanks to the higher buffer volume transfer times of more than 24 h can be realised, e. g. for gentle blotting of very big proteins. Different models are available: The highly sophisticated Tankblot for mini-gels offers the most effective cooling by the laterally integrated cooling jacket. Tankblot Eco-Mini and Tankblot Eco- Maxi are part of the modular Eco-Line and offer highly effective cooling of small and large gels by integrated cooling bases. The Mini-V8·10 Blot Module is designed for use in the buffer tank of the Mini-V8·10 vertical gel electrophoresis apparatus.

Tankblot
In the Tankblot a cooling jacket is integrated into the sidewalls. This results in convective chilling by circulation: the temperature is distributed more homogeneously (± 0.5 °C at 6 °C buffer temperature) than with other designs that use a magnetic stirrer circulating the buffer. Additionally this special design prevents formation of ion gradients without the need for a magnetic stirrer. Depending on the selected buffer, transfer can be fast (between 30 min. and 4 h) or, under exceptionally gentle conditions, overnight (ca. 12 h). Thanks to the buffer volume of 1,200 ml long transfer times can be realised.

Tankblot Eco-Line
Wire electrodes are placed on the side walls to provide uniform, reproducible protein transfer over a wide molecular weight range. The lower buffer chamber with built-in cooling base absorbs heat generated during rapid transfers and allows temperature controlled runs. A stir bar may be added to the buffer chamber to further improve buffer circulation and heat exchange. The colour coded blotting cassettes in combination with the colour coded connectors for the Bigfoot Safety Lid ensure accurate orientation of the gel sandwich during the transfer. The tankblot is available as stand-alone apparatus or as a module compatible with the buffer chamber EBC (with integrated cooling base) or EB (without cooling option).

The Electrophoresis Module for Eco-Mini and Eco-Maxi are available separately and will convert the corresponding tank blotting systems into powerful PAGE electrophoresis systems.

Tankblot Eco-Mini
Tankblot Eco-Mini is designed to transfer up to four PAGE mini-gels (up to 9.5 cm x 8.5 cm) in separate blotting cassettes simultaneously. The special design of the blotting cassettes allows easy assembly and loading into the blot module. Additional the assembly defends blotting sandwiches from squeezing.

Tankblot Eco-Maxi
Tankblot Eco-Maxi is the large version for gel sizes up to 22 cm x 19 cm. The system offers the capacity of multiple transfers: several small gels or 1 large gel per cassette. Up to 2 large gels can be transferred simultaneously in separate blotting cassettes. The special design for fixing the blotting sandwich in the blotting cassette prevents squeezing and allows easy handling.

 

Technical Data

Compatibility of gel sizes

Tankblot Eco-Mini

Tankblot Eco-Maxi

Blotting area (W x L, cm)

9.4 cm × 8.0 cm

22.0 cm × 19.0 cm

Minigel

+

+

Minigel-Twin

+

+

Eco-Mini

+

+

Mini-V8.10

+

+

Multigel

-

+

Multigel-Long

-

+

Modell V15.17

-

+

Maxigel

-

+

Eco-Maxi

-

+

+ = recommended

(+) = limited use

- = not recommended

Order Information

Order numberItem
 Order Information Tankblot Eco-Mini
846-018-100Tankblot Eco-Mini C, complete instrument with integrated cooling base, Blot Module, 4 Blotting Cassettes and 8 foam pads
846-018-101Tankblot Eco-Mini, complete instrument (without cooling base), Blot Module, 4 Blotting Cassettes and 8 foam pads
 Order Information Tankblot Eco-Maxi
846-018-400Tankblot Eco-Maxi C, complete system with buffer chamber EBC (with integrated cooling base), Bigfoot Safety Lid, 2 Blotting Cassettes (black/red), each for 1 gel with size 22 cm x 19 cm, 4 foam pads
846-018-401Tankblot Eco-Maxi, complete system with buffer chamber EB (without cooling base), Bigfoot Safety Lid, 2 Blotting Cassettes (black/red), each for 1 gel with size 22 cm x 19 cm, 4 foam pads
Order numberAccessories
 Accessories Tankblot
846-013-301Gel cassette (blotting cassette) for up to four 10 cm x 10 cm gels, 1/pkg
846-013-304Fixation rings for gel cassette, 4/pkg
846-013-305Fiber pads, 4/pkg
 Accessories Tankblot Eco-Mini
846-017-170Eco-Mini buffer chamber (with integrated cooling base), without safety lid
846-017-171Eco-Mini safety lid
846-017-175Electrophoresis Module for Eco-Mini (2 gels)
846-018-105Blot Module Eco-Mini, incl. 4 Blotting Cassettes
846-018-111Blotting Cassette, 1/pkg
846-018-113Foam pads, 4/pkg
 Accessories Tankblot Eco-Maxi
846-017-471Buffer chamber EB (without cooling base) for Eco-Maxi and Tankblot Eco-Maxi, without Bigfoot Safety Lid
846-017-472Buffer chamber EBC (with integrated cooling base) for Eco-Maxi and Tankblot Eco-Maxi, without Bigfoot Safety Lid
846-017-474Bigfoot Safety Lid for Eco-Maxi and Tankblot Eco-Maxi
846-017-475Electrophoresis Module for Eco-Maxi (2 gels)
846-018-405Blot Module Eco-Maxi for up to 2 Blotting Cassettes (black/red), each for 1 gel with size 22 cm x 19 cm and 4 foam pads
846-018-411Blotting Cassette, colour coded (black/red), for Tankblot Eco-Maxi Blot Module, 1/pkg
846-018-413Foam pads for Tankblot Eco-Maxi Blotting Module, 4/pkg
 Blotting Paper
846-B3030931Whatman 3MM Chr, 580 mm x 680 mm, 0.34 mm thick, 100/pkg
846-B3017915Whatman 17 Chr, 460 mm x 570 mm, 0.92 mm thick, 25/pkg
846-B10 426 981Whatman GB005, 200 mm x 200 mm, 1.5 mm thick, 25/pkg
846-B10 426 994Whatman GB005, 580 mm x 580 mm, 1.5 mm thick, 25/pkg

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