RoboGene MTB DNA Qualitative Kit

Efficient TaqMan-based Assay for detection of bacterial strains M. tuberculosis and M. bovis using quaitative real-time PCR

Product Details

  • Contains ready-to-use, lyophilized standards and extraction tubes coated with Internal Control DNA
  • Highly sensitive despite minimal sample input volume (10 copies/ml)
  • Full Volume Reagent Guarantee for processing of 50 or 100 clinical samples excluding positive controls

The RoboGene® MTB Qualitative Kit is an amplification test for detection of MTB DNA in human sputum, bronchalveolar lavage, or e.g. lymph node samples. Both M. tuberculosis and M. bovis are amplified with equal efficiency applying primers and probe sets specific for the insertion sequence IS6110 and the MTB genome. The qualitative standard consists of tubes coated with a known amount of synthetic MTB DNA, which must be amplified in parallel with the samples. A synthetic internal control (IC) to monitor the complete procedure is included via extraction tubes. Amplification of MTB DNA in samples and standards and of IC DNA is measured independently at different wavelengths due to probes labelled with different fluorescence reporter dyes (MTB DNA: FAM channel; IC DNA: Yakima Yellow/VIC/JOE channel). Regarding the plastic ware the kit is available in a variety of configurations depending on the real-time PCR device. For sample preparation the “INSTANT Mycobacterium DNA Kit” (AJ Innuscreen) is recommended. 

Specifications

Starting Material

Human sputum, bronchalveolar lavage, or e.g. lymph node samples
 

Detection principle

real-time PCR (TaqMan)

Target

insertion sequence IS6110

Order Information

Order number    Quantity
847-0207300502
847-0207300504
Regular profile strips
0.2 ml clear (RUO)
128 reactions
72 reactions
847-0207300542
847-0207300544
Regular profile tubes
0.2 ml clear (RUO)
120 reactions
60 reactions
847-0207300552
847-0207300554
Reaction tubes 25 µl
(RUO)
120 reactions
60 reactions
847-0207300562
847-0207300564
Low profile strips
0.1 ml clear (RUO)
128 reactions
72 reactions
847-0207300582
847-0207300584
Low profile strips
0.1 ml white (RUO)
128 reactions
72 reactions

 

Manufacturer:

AJ Roboscreen GmbH
Hohmannstrasse 7
04129 Leipzig
Germany
Phone: +49 341 989734 0
Fax: +49 341 989734 199

Downloads

Manual RoboGene MTB DNA Qualitative Kit

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