RoboGene HCMV DNA Quantification Kit

Real-time PCR-based detection system for quantification of HCMV-DNA

Product Details

  • The RoboGene HCMV DNA Quantification Kit is intended for real-time PCR quantification of Human Cytomegalovirus (HCMV) DNA in human EDTA plasma or serum samples
  • The RoboGene HCMV DNA Quantification Kit is intended for research use only but not for diagnostic procedures!
  • Reliable quantification due to HCMV- and IC-DNA pre-coated lyophilized standard tubes
  • Highly sensitive despite minimal sample input volume
  • including internal extraction control
  • Full Volume ReagentGuarantee for processing of 50 or 100 samples excluding positive controls

The RoboGene HCMV DNA Quantification Kit is an amplification test for quantification of Human Cytomegalovirus (HCMV) DNA in human plasma or serum samples. The Kit contains quantitation standard which consists of 8 tubes coated with given amounts of synthetic HCMV DNA that must be amplified in parallel. A synthetic internal control is included via Extraction tubes to monitor the whole procedure. Regarding the plastic ware the kit is available in a variety of configurations depending on the real-time PCR device. Amplification of HCMV DNA in samples and standards and of IC DNA is measured independently at different wavelengths due to probes labeled with different fluorescence reporter dyes (HCMV DNA: FAM channel, IC DNA: Yakima Yellow channel). For sample preparation the INSTANT Virus RNA Kit or INSTANT Virus RNA/DNA Kit are recommended. For final elution of filter bound DNA the use of 60 µl of elution buffer is suggested.

Specifications

Starting material

Human plasma and serum

Extraction time

Approx. 25 minutes

Detection principle

real-time PCR (TaqMan)

Order Information

Order number Quantity
847-0207500202
847-0207500204

Regular profile strips
0.2 ml clear (RUO)

136 reactions
72 reactions
847-0207500242
847-0207500244
Regular profile tubes
0.2 ml clear (RUO)
132 reactions
66 reactions
847-0207500262
847-0207500264
Low profile strips
0.1 ml clear (RUO)
136 reactions
72 reactions
847-0207500282
847-0207500284
Low profile strips
0.1 ml white (RUO)
136 reactions
72 reactions

 

Manufacturer:

AJ Roboscreen GmbH
Hohmannstrasse 7
04129 Leipzig
Germany
Phone: +49 341 989734 0
Fax: +49 341 989734 199

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