RoboGene HCMV DNA Quantification Kit

Real-time PCR-based detection system for quantification of HCMV-DNA

Product Details

  • Recommended for supporting diagnosis or assessing the responds to antiviral treatment following In-house validation
  • Reliable quantification due to HCMV- and IC-DNA pre-coated lyophilized standard tubes
  • Highly sensitive despite minimal sample input volume
  • including internal extraction control
  • Full Volume ReagentGuarantee for processing of 50 or 100 clinical samples excluding positive controls

The RoboGene HCMV DNA Quantification Kit is an amplification test for quantification of Human Cytomegalovirus (HCMV) DNA in human plasma or serum samples. The Kit contains quantitation standard which consists of 8 tubes coated with given amounts of synthetic HCMV DNA that must be amplified in parallel. A synthetic internal control is included via Extraction tubes to monitor the whole procedure. Regarding the plastic ware the kit is available in a variety of configurations depending on the real-time PCR device. Amplification of HCMV DNA in samples and standards and of IC DNA is measured independently at different wavelengths due to probes labeled with different fluorescence reporter dyes (HCMV DNA: FAM channel, IC DNA: Yakima Yellow channel). For sample preparation the INSTANT Virus RNA Kit or INSTANT Virus RNA/DNA Kit are recommended. For final elution of filter bound DNA the use of 60 µl of elution buffer is suggested.


Starting material

Human plasma and serum

Extraction time

Approx. 25 minutes

Detection principle

real-time PCR (TaqMan)

Order Information

Order number Quantity

Regular profile strips
0.2 ml clear (RUO)

136 reactions
72 reactions
Regular profile tubes
0.2 ml clear (RUO)
132 reactions
66 reactions
Low profile strips
0.1 ml clear (RUO)
136 reactions
72 reactions
Low profile strips
0.1 ml white (RUO)
136 reactions
72 reactions

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