RoboGene HBV DNA Quantification Kit 3.0

Real-Time PCR-based detection system for highly sensitive quantification of Hepatitis B-Virus (HBV)-DNA

Product Details

  • CE-IvD marked kit for quantitative detection of Hepatitis B virus (HBV) DNA
  • Detection of genotypes A-I inclusive subtypes
  • Validated for manual and automated nucleic acid extraction methods
  • precise results due to 4 included lyophilised quantification standards

The RoboGene HBV DNA Quantification Kit 3.0 is validated for quantification of Hepatitis B Virus DNA in human plasma or serum samples using One-Step Real-Time PCR. The universal kit setup allows flexible and reliable application to most major Real-Time PCR platforms currently available on the market.

The Internal Control is provided as an IC Spiking Tube for convenient handling with manual as well as automated extraction methods. Due to probes labeled with different fluorescent reporter dyes, amplification of HBV DNA and IC in samples and standards are measured independently at different wavelengths (HBV in FAM channel, IC in VIC or Cy5 channel). Benefit from superior sensitivity and specificity and rely on defined lyophilized standards covering concentrations from 2.5x102 to 2.5x107 IU/mL for precise data acquisition. Three kit sizes, all including 4x4 standards, are available to provide the perfect solutions from low to high throughput laboratories.

Specifications

Starting Material

DNA from human plasma and serum

Target

Hepatitis B surface antigen (HBsAg)

Detection principle

Quantitative qPCR (TaqMan®)

Linear range

9 IU/mL - 2.5x109 IU/mL

Validated PCR platforms

qTOWER³ (AJ), CFX 96 (Biorad), LightCycler® 480 II (Roche), Rotor-Gene® 3000/6000/Q (Corbett Research/ Qiagen), 7500 Fast (ABI) 

Detection time

<120 min

Sensitivity

7.3 IU/mL (manual extraction)

6.1 IU/mL (automated extraction)

Order Information

Order numberQuantity
847-0207710032
 
32 reactions
 
847-0207710096
 
96 reactions
 
847-0207710192
 
192 reactions
 

 

Manufacturer:

AJ Roboscreen GmbH
Hohmannstrasse 7
04129 Leipzig
Germany
Phone: +49 341 989734 0
Fax: +49 341 989734 199

Downloads

IFU_RoboGene_HBV DNA Quant Kit 3.0

Open PDF

mdc medical device certification GmbH

Open PDF

Br_RoboGene Family_en

Open PDF

Contact us We are happy to help you and will contact you as quickly as possible.