smart Blood DNA Midi prep (a96) - FX

Extraction of high molecular weight DNA based on unique SmartExtraction Technology

Product Details

  • Up to 90 µg DNA from of up to 3 ml whole blood
  • Excellent performance tested for extraction of DNA from fresh or frozen blood stabilized with EDTA, citrate and heparin, as well as blood sampled with PAXgene® Blood DNA Tube
  • High yield of large DNA fragments based on innovative SmartExtraction technology
  • Minimal preparation thanks to pre-filled reagents

The smart Blood DNA Midi prep (a96) – FX kit has been designed for automated isolation of high molecular weight genomic DNA from peripheral blood mononuclear cells (PBMC) derived from fresh or frozen blood using CyBio FeliX. The kit can handle sample volumes of up to 3 ml, whereby blood samples can be stabilized with EDTA, citrate, heparin as well as with PAXgene® Blood DNA Tubes. The extraction method combines the innovative SmartExtraction technology developed by Analytik Jena (patent pending) and the advantages of low-salt DC-Technology (patented). After erythrocyte lysis the PBMCs are pelleted. Subsequently, PBS is added and the cells are resuspended and transferred into the kit’s Reagent Plate. The whole extraction process just needs simple pipetting up and down and profits from efficient binding of genomic DNA to Smart Modified Surfaces inside the filter tip, which minimizes DNA fragmentation. After washing steps, the high molecular weight DNA (up to 350 kbp) is eluted and ready for use in subsequent downstream applications.

By choosing the premium pre-filled kit version, hands-on time is minimized and handling vastly simplified. However, low-priced non-filled kit versions are available, too.

 

SmartExtraction - We Change the Way to Prep

Nucleic acid isolation and purification are among the most widespread laboratory methods worldwide. Analytik Jena's innovative “SmartExtraction” technology is a global innovation in nucleic acid extraction.

Specifications

Starting Material

  • 0.5 – 3 ml whole blood
  • Fresh or frozen
  • stabilized with EDTA, citrate or heparin
  • PAXgene® Blood DNA Tube

Average Yield

  • Depending on sample quality/amount of starting material
  • 0.5 ml whole blood: 5 – 15 µg
  • 1.0 ml whole blood: 15 – 30 µg
  • 2.0 ml whole blood: 40 – 70 µg
  • 3.0 ml whole blood: 50 – 90 µg

 

Volume

150 – 500 µl

Extraction Time

  • External Lysis: approx. 40 min
  • Extraction: 40 min

Order Information

Order number  Amount
845-FX-419609696 reactions (Non-filled)
845-FX-41964805 x 96 reactions (Non-filled)
845-PFX-419609696 reactions (Pre-filled)
This kit can only be used in CyBio FeliX> using the CyBio Extraction Set.

OL5015-25-120

                                   

CyBio Extraction Set
(includes: 1x CyBio Felix head R 96/1000 µl, incl. protective magazine; 3x 96 channel magazine for CyBio Felix head R 96/1000 µl; 3x holder; 97 mm height; 1x 8 channel adapter for CyBio Felix head R 96/1000 µl incl. holder; 37 mm height; 1x CyBio FeliX gripper incl. holder; 37 mm height; 1x magnetic adapter Magnum FLX Enhanced; 1x BioShake 3000-T elm, incl. adapter for deep well plates; 1x waste box; 1x tip converter 96/1000 µl; 1x tip rack 96/1000 µl; control accessories (cable, USB hub, adapter))

Downloads

Manual smart Blood DNA Midi prep (a96) - FX,prefilled

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Manual smart Blood DNA Midi prep (a96) - FX

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SDS smart Blood DNA Midi prep (a96) - FX

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SDS smart Blood DNA Midi prep (a96) - FX_prefilled_en_20190620

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Automated DNA/RNA Extraction with the CyBio FeliX

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Brochure SmartExtraction (eng)

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CyBio FeliX

Is time of the essence? Is your laboratory space limited? Would you like to save on costs? Then we have the pipetting platform for you. Optimize your work processes with CyBio FeliX, a liquid handling system adaptable to your requirements. CyBio FeliX is a flexible pipetting platform for fully automated single- to multichannel liquid handling tasks.

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