smart Blood DNA Midi prep (a)

Efficient DNA extraction from PBMC's (Peripheral Blood Mononuclear Cells) from fresh or frozen whole blood

Product Details

  • Suitable for all common stabilizers: EDTA, citrate and heparin
  • Based on SmartExtraction technology for easy automation without any additional tools needed
  • Optimized for InnuPure systems, GeneTheatre, CyBio FeliX
  • Isolation of high molecular DNA with a yield up to 90 μg from 3 ml whole blood
  • Inclusive pre-filled Reagent Strips or Plates for simple handling and a minimum number of working steps

The smart Blood DNA Midi prep (a) is based on the unique SmartExtraction technology and allows an easy automation of DNA extraction from up to 3 ml of whole blood. Initially erythrocytes are lysed and PBMCs are collected for further preparation. Pre-filled, sealed Reagent Plastics simplifies handling enormously and can be applied on InnuPure systems, GeneTheatre, or CyBio FeliX directly. Additional tools are not necessary. Possible shearing effects on DNA are reduced to an absolute minimum due to the special SmartExtraction pipette tips, which contains modified surfaces for efficient binding of nucleic acids. Finally high DNA yields (up to 90 μg from 3 ml whole blood) with high molecular weight (up to 500 kbp) can be isolated. An excellent quality ensures the direct application in further downstream analyses.

SmartExtraction - We Change the Way to Prep

Nucleic acid isolation and purification are among the most widespread laboratory methods worldwide. Analytik Jena's innovative “SmartExtraction” technology is a global innovation in nucleic acid extraction.

Specifications

Average Yield

Depends on sample and used volume

  • 0.5 ml whole blood: approx. 5 – 15 μg gDNA
  • 1.0 ml whole blood: approx. 15 – 30 μg gDNA
  • 2.0 ml whole blood: approx. 40 – 70 μg gDNA
  • 3.0 ml whole blood: approx. 50 – 90 μg gDNA

Starting Material

  • Whole blood samples of 0.5 to 3 ml
  • Fresh or frozen blood
  • Stabilizers: EDTA, citrate or heparin

Extraction Time

  • Lysis of erythrocytes: approx. 20 min.
  • Automatic processing: approx. 40 to 90 minutes (depending on protocol and device)

Quality

1.7 – 2.0

Volume

200 – 500 μl Elution Buffer

Order Information

Order number AmountCompatibility
845-ASS-1208016                                          16 reactions, Reagent Strips,
1 reaction per Strip
InnuPure C16GeneTheatre,CyBio FeliX
845-ASS-120809696 reactions, Reagent Strips,
1 reaction per Strip
InnuPure C16GeneTheatreCyBio FeliX
845-ASP-120801616 reactions, Reagent Plates, 8 reactions per PlateInnuPure C16GeneTheatreCyBio FeliX
845-ASP-120809696 reactions, Reagent Plates, 8 reactions per PlateInnuPure C16GeneTheatreCyBio FeliX
845-ASP-12960961 x 96 reactions, Reagent PlatesInnuPure C96CyBio FeliX

Downloads

Application Note SmartExtraction nucleic acid extraction multiple samples

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Manual smart Blodd DNA Midi Direct a

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Product Brochure SmartExtraction

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