innuSPEED Bacteria/Fungi DNA Kit

Optimum isolation of genomic DNA from gram+ bacteria, yeast and fungal spores 

Product Details

  • Includes Lysis Tubes with application-specific beads for highly efficient homogenization with a SpeedMill (or other homogenizers)
  • Effective sample disruption for extremely high DNA yields
  • High-quality DNA; free of inhibitors

The innuSPEED Bacteria/Fungi DNA Kit has been specially devel-oped for isolating genomic DNA from gram+ bacteria, fungal spores and yeasts. The extraction protocol combines two steps: rapid homogenization of these hard-to-access starting materials, and efficient digestion of microbial cell walls in specially developed Lysis Tubes (using a SpeedMill or other homogenizer). The material is first broken down mechanically and then subjected to thermal and enzymatic lysis. The DNA is then selectively bound to the surface of to remove inhibitors, and then by addition of a low-salt buffer to separate bound DNA from the membrane.

Specifications

Starting material

  • Gram+ bacteria (max. 1 ×  109)
  • Fungal spores
  • Yeasts  (max.  1 ×  109)

Average yield

Depends on the type and quantity of the starting material used; up to 30 µg

Extraction time

  • Homogenization and lysis: approx. 30 – 45 minutes
  • Purification: approx. 12 minutes

Binding capacity

Column binding capacity: > 30 µg DNA

Average purity

1,7 – 2,0

Order Information

Order numberQuantity
845-KS-151001010 reactions
845-KS-151005050 reactions

Downloads

Brochure Product Guide Extraction

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Manual innuSPEED Bacteria/Fungi DNA Kit

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MSDS innuSPEED Bacteria Fungi DNA

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Other Devices

SpeedMill PLUS

The SpeedMill PLUS is a highly efficient homogenization system for various starting materials used for the subsequent isolation and purification of DNA, RNA, or proteins.

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