innuPREP FFPE total RNA Kit

  • Isolation of the ideal integrity and quality RNA from FFPE tissue samples
  • Total RNA including high quantities of microRNAs

Product Details

  • Extraction process finished within less than 1.5 hour
  • RNA free of impurities – no residues of paraffin
  • Excellent formaldehyde-RNA decrosslinking
  • High amounts of tissue in starting material (up to 50 mg)
  • Deparaffinization step excluded: no use of hazardous or toxic components like phenol, octane or xylene
  • Extracted RNA (including microRNA) can be used for a wide range of different molecular biology applications

The innuPREP FFPE total RNA Kit allows the extraction of total RNA with an excellent recovery of microRNA’s from FFPE (formalin-fixed, paraffin-embedded) tissue samples. The kit is suitable for starting amounts of up to 50 mg. Based on a new kind of chemistry the kit enables the isolation of RNA from FFPE samples without deparaffinization step and thereby excludes the use of toxic and hazardous components like octane or xylene. After 1.5 h and only lysis, binding, washing, elution the whole extraction routine is finished. Thus the protocol allows the extraction of RNA with high quality, ideal integrity and excellent formaldehyde- RNA decrosslinking The isolated RNA including microRNA’s is directly suitable for reverse transcription, amplification reaction and other amplification based downstream applications.

Specifications

Starting Material

  • FFPE (formalin fixed paraffin embedded) tissue samples
  • Maximum amount of tissue: 50 mg

Extraction Time

Less than 1.5 hours (incl. lysis)

Binding Capacity

> 50 μg total RNA

Average Purity

1,7 – 2,2

Average Yield

Depends on the type and starting quantity of the FFPE tissue material

Order Information

Order numberQuantity
845-KS-205001010 reactions
845-KS-205005050 reactions

Downloads

Brochure Product Guide Extraction

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Manual innuPREP FFPE total RNA Kit

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MSDS innuPREP FFPE total RNA Kit

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