innuPREP Blood DNA Mini Kit - FX

Ready-to-use application for extraction of genomic DNA

Product Details

  • Automated procedure including lysis
  • High throughput application for up to 96 samples in parallel
  • Diverse starting material: fresh or frozen whole blood samples stabilized with EDTA, citrate, heparin or collected in PAXgene® Blood DNA Tubes
  • Flexible application: protocol for 200 µl and 400 µl
  • High yield and high purity DNA

The innuPREP Blood DNA Mini Kit-FX has been designed for automated extraction of genomic DNA (gDNA) from whole blood preparations including fresh or frozen whole blood samples stabilized with EDTA, citrate or heparin and even collected in PAXgene® Blood DNA Tubes. The extraction protocol is established on CyBio FeliX based on sample volumes of up to 400 µl. The extraction process is based on binding the nucleic acids to magnetic particles. The procedure starts with the lysis of the starting material. After washing steps the nucleic acid is eluted from the magnetic particles with RNase-free water. The protocol is optimized to obtain DNA of maximum yield and quality which can be used for downstream applications like standard or real-time PCR. The extraction procedure is based on the Dual-Chemistry (DC) technology patented by Analytik Jena.
In order to minimize manual preparation steps for this kit the innuPREP Prefilling Set (845-FX-3000005) offers the possibility of automated prefilling of all Deep Well Plates with respective buffers and reagents by the CyBio FeliX.

Specifications

Starting material

  • Up to 400 µl whole blood
  • Fresh or frozen blood
  • Stabilized with EDTA, citrate or heparin
  • PAXgene® Blood DNA Tubes

Average yield

  • Depending on sample quality/amount of mononuclear blood cells
  • 200 µl whole blood approx. 2 – 8 µg
  • 400 µl whole blood approx. 2 – 12 µg

Extraction time

  • Up to 200 µl whole blood: 70 min
  • Up to 400 µl whole blood: 86 min

Average purity

1.8 – 2.0

Order Information

Order numberQuantity
845-FX-229609696 reactions
845-FX-2296480480 reactions
This kit can only be used in CyBio FeliX using the CyBio Extraction Set.
OL5015-25-120                                CyBio Extraction Set
(includes: 1x CyBio Felix head R 96/1000 µl, incl. protective magazine; 3x 96 channel magazine for CyBio Felix head R 96/1000 µl; 3x holder; 97 mm height; 1x 8 channel adapter for CyBio Felix head R 96/1000 µl incl. holder; 37 mm height; 1x CyBio FeliX gripper incl. holder; 37 mm height; 1x magnetic adapter Magnum FLX Enhanced; 1x BioShake 3000-T elm, incl. adapter for deep well plates; 1x waste box; 1x tip converter 96/1000 µl; 1x tip rack 96/1000 µl; control accessories (cable, USB hub, adapter))

Downloads

Manual innuPREP Blood DNA Mini Kit - FX

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SDS innuPREP Blood DNA Mini Kit FX

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Automated DNA/RNA Extraction with the CyBio FeliX

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Other Devices

CyBio FeliX

Is time of the essence? Is your laboratory space limited? Would you like to save on costs? Then we have the pipetting platform for you. Optimize your work processes with CyBio FeliX, a liquid handling system adaptable to your requirements. CyBio FeliX is a flexible pipetting platform for fully automated single- to multichannel liquid handling tasks.

Learn more: CyBio FeliX

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