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Analytik Jena > Products bio solutions > Kits | nucleic acid isolation (Article ID 2251)  



Kits for nucleic acid isolation


Products for genomic DNA isolation - Spin Filter based
Plasmid purification and cleanup products
 
Isolation of total RNA
Isolation of microbial DNA or RNA
Magnetic particle based nucleic acid isolation
Nucleic acid isolation using a homogenizer
Costum-made Products


Genomic DNA isolation - Spin Filter based

The innuPREP DNA Kits are determined for very fast and efficient isolation of genomic DNA from different types of starting materials. The extraction procedure is based on a new kind of chemistry, which combines an extreme fast lysis step with a subsequent efficient binding of genomic DNA on a Spin Filter surface following washing of the bound DNA and finally eluting of the DNA. The recovery of DNA and the quality are excellent.

Description
Size
Unit

Order-No.

 
innuPREP DNA Micro Kit

For very fast and efficient isolation of genomic DNA from small amounts of different types of starting materials like microbiopsies, whole blood up to 50 µl, blood sticks and from limited amounts of cells.

10
50
250

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845-KS-1010010
845-KS-1010050
845-KS-1010250

 
innuPREP DNA Mini Kit

For very fast and efficient isolation of genomic DNA from different amounts and different types of starting materials like tissue samples up to 50 mg, paraffin embedded tissue material, buccal swabs, mouse or rodent tail, eucaryotic cell pellets.

10
50
250

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845-KS-1040010
845-KS-1040050
845-KS-1040250

 
innuPREP Forensic Kit

For very fast and efficient isolation of genomic DNA from small amounts of different types of forensic samples like, hairs or hair roots; stains of blood, saliva or sperm; finger nails; cigarette butts; bubble gum; buccal swabs; stamps and envelopes as well as fingerprints on different surfaces. The kit is a very efficient tool for isolation of DNA for application where only limited amounts of starting material and very dirty material is available. Further, the new kind of extraction chemistry allows particularly the recovery of degraded DNA.

10
50
250

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845-KS-1050010
845-KS-1050050
845-KS-1050250

 
innuPREP Blood DNA Mini Kit
For isolation of genomic DNA direct from whole blood samples
from 1 µl up to 250 µl.

10
50
250

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845-KS-1020010
845-KS-1020050
845-KS-1020250

 
innuPREP Blood DNA Midi Kit

For isolation of genomic DNA from whole blood samples from 0.5 ml up to 2.0 ml. The kit combines the selective lysis of erythrocytes, pelleting of nucleus containing blood cells and subsequent lysis of the cells and protein removing following binding of the genomic DNA on a Spin Filter surface and elution of the bound DNA.

10
50
250

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845-KS-1030010
845-KS-1030050
845-KS-1030250

 
innuPREP Plant DNA Kit

For isolation of genomic DNA from up to 100 mg of different kinds of plant material. The kit combines lysis of starting material, filtration of unlysed components and subsequent binding of genomic DNA on a Spin Filter surface. After washing the bound DNA will be eluted from the Spin Filter.

10
50
250

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845-KS-1060010
845-KS-1060050
845-KS-1060250


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Plasmid purification and cleanup products

Description
Size
Unit

Order-No.

 
 innuPREP Plasmid Mini Kit
For isolation of plasmid DNA from 1 - 5 ml bacteria suspension.

10
50
250

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845-KS-5040010
845-KS-5040050
845-KS-5040250

 
innuPREP PCRpure Kit

For fast and efficient purification of PCR products from PCR reaction mixtures based on a new two-step-procedure (bind and elute). No washing steps are necessary.

10
50
250

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845-KS-5010010
845-KS-5010050
845-KS-5010250

 
innuPREP Gel Extraction Kit

For extraction and recovery of DNA-fragments from TAE or TBE-agarose gels.

10
50
250

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845-KS-5030010
845-KS-5030050
845-KS-5030250

 
innuPREP DOUBLEpure Kit
For fast and efficient purification of PCR products from PCR reaction mixtures based on a new two-step-procedure and/or for the extraction and recovery of DNA-fragments from TAE or TBE-agarose gels.

10
50
250

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845-KS-5050010
845-KS-5050050
845-KS-5050250

 
innuPREP DYEpure Kit

For fast removing of dye terminators from sequencing mixes based on a new two-step-procedure (bind and elute). No washing steps are necessary. No ethanol precipitation or sephadex.

10
50
250

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845-KS-5020010
845-KS-5020050
845-KS-5020250



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Isolation of total RNA

The innuPREP RNA Kits are developed for very fast and efficient isolation of cellular Total RNA. The kits combines lysis of starting material following a step for selective removing of genomic DNA without DNase I. After removing of genomic DNA the RNA will be bound onto a Spin Filter surface, washed and eluted. The extracted RNA is from excellent quality and useful for all common downstream applications.

Description
Size
Unit

Order-No.

 
innuPREP RNA Mini Kit

For very fast and efficient isolation of cellular Total RNA from eucaryotic cells, bacteria and tissue samples.

10
50
250

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845-KS-2040010
845-KS-2040050
845-KS-2040250

 
innuPREP Blood RNA Kit
For very fast and efficient isolation of cellular Total RNA from 0.5 ml - 1.0 ml of whole blood.

10
50
250

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845-KS-2010010
845-KS-2010050
845-KS-2010250

 
innuPREP Plant RNA Kit
For very fast and efficient isolation of cellular Total RNA from different types of plant material.

10
50
250

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845-KS-2060010
845-KS-2060050
845-KS-2060250


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Isolation of microbial DNA or RNA

The INSTANT Kit Technology
All Kits of INSTANT series contains special Extraction Tubes, which are coated by a storage stable Carrier Nucleic Acids and a storage stable Internal Extraction Control. This easily gives answer about extraction quality and efficiency. Moreover it helps to avoid the problem of false negative results based on inhibitors or other negative causes.

Description
Size
Unit

Order-No.

 
INSTANT Bacteria DNA Kit

For isolation of DNA from pellets of gram+ and gram- bacteria after cultivation. The extraction procedure combines a lysis step with lysozym following Proteinase K digestion and subsequent efficient binding of genomic DNA on a Spin Filter surface. The bound DNA will be washed and eluted.

10
50
250

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845-KS-6050010
845-KS-6050050
845-KS-6050250

 
INSTANT Mycobateria DNA Kit

For isolation of DNA from Mycobacteria containing sputum, bronchoalveolar lavage and lymph note samples. The extraction procedure combines optional a sample pretreatment with
N-actetylcysteine, pelleting of bacteria and subsequent bacteria lysis with lysozym following Proteinase K digestion and subsequent efficient binding of the DNA on a Spin Filter surface. The bound DNA will be washed and eluted.

10
50
250

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845-KS-6150010
845-KS-6150050
845-KS-6150250

 
INSTANT Virus DNA Kit

For isolation of viral DNA from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs and from cell cultures. The extraction procedure is based on a new kind of chemistry, which combines an extreme fast lysis step with a subsequent efficient binding of genomic DNA on a Spin Filter surface following washing of the bound DNA and finally eluting of the DNA.

10
50
250

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845-KS-4150010
845-KS-4150050
845-KS-4150250

 
INSTANT Virus RNA Kit

For isolation of viral RNA from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs and from cell cultures.

10
50
250

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845-KS-4250010
845-KS-4250050
845-KS-4250250

 
INSTANT INFLUENZA RNA Kit

For very fast isolation of INFLUENZA RNA from primary samples of tracheal swabs or organ grip stored under medium (e.g. enrichment medium). After sample lysis the viral RNA will be bound onto a spin filter surface following washing steps and finally eluting of the bound RNA.

10
50
250

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845-KS-4350010
845-KS-4350050
845-KS-4350250

 
innuPREP Bacteria DNA Kit

For isolation of DNA from pellets of gram+ and gram- bacteria after cultivation. The extraction procedure combines a lysis step with lysozym following Proteinase K digestion and subsequent efficient binding of genomic DNA on a Spin Filter surface. The bound DNA will be washed and finally eluted.

10
50
250

Reactions
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845-KS-6000010
845-KS-6000050
845-KS-6000250

 
innuPREP Stool DNA Kit

For isolation of bacterial DNA from stool samples. The kit combines a very efficient lysis of starting materials following a prefiltration step to remove unlysed particle. After prefiltration the sample will be transferred onto a spin filter unit and the DNA will be bound on the Spin Filter surface. After washing of the bound DNA the DNA will be eluted from the Spin Filter and is ready to use for further downstream applications.

10
50
250

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845-KS-7000010
845-KS-7000050
845-KS-7000250

 
innuPREP Mycobacteria DNA Kit

For isolation of DNA from Mycobacteria containing sputum, bronchoalveolar lavage and lymph note samples. The extraction procedure combines optional a sample pretreatment with
N-actetylcysteine, pelleting of bacteria and subsequent bacteria lysis with lysozym following Proteinase K digestion and subsequent efficient binding of the DNA on a Spin Filter surface. The bound DNA will be washed and eluted.

10
50
250

Reactions
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845-KS-6100010
845-KS-6100050
845-KS-6100250


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Magnetic particle based nucleic acid isolation

Description
Size
Unit
        Order-No.
 
innuPREP DNA Kit I-KFml

For isolation of genomic DNA from whole blood and tissue sample and for isolation of bacterial DNA from urine and urogenital swabs using KingFisher mL processor.

250

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845-KF-8015250

 
innuPREP Bacteria DNA Kit-KFml

For isolation of DNA from pellets of gram+ and gram- bacteria after cultivation using KingFisher mL processor.

250

Reactions

845-KF-6015250

 

The INSTANT Kit Technology
All Kits of INSTANT Virus series contains special Extraction Tubes, which are coated by a storage stable Carrier Nucleic Acid and a storage stable Internal Extraction Control. This easily gives answer about extraction quality and efficiency. Moreover it helps to avoid the problem of false negative results based on inhibitors or other negative causes.

 
INSTANT Virus DNA Kit-KFml

For isolation of viral DNA from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs and from cell cultures using KingFisher mL processor.

250

Reactions

845-KF-4115250

 
INSTANT Virus RNA Kit-KFml

For isolation of viral RNA from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs and from cell cultures using KingFisher mL processor.

250

Reactions

845-KF-4215250

 
INSTANT INFLUENZA RNA Kit-KFml

For very fast isolation of INFLUENZA RNA from primary samples of tracheal swabs or organ grip stored under medium (e.g. enrichment medium) using KingFisher mL processor.

250

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845-KF-4315250

 
innuPREP Stool DNA Kit-KF96

For isolation of bacterial DNA from stool samples using KingFisher 96 processor.

96
480

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845-KF-7015096
845-KF-7015480


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Nucleic acid isolation using a homogenizer

innuSPEED DNA Kit
For extreme fast and efficient isolation of DNA from different starting materials. The kit contains specialized Lysis Tubes, which will be processed in a homogenizer. The Lysis Tubes contain further an optimized buffer component, which is compatible with the further isolation of DNA. The mechanical disruption of starting material and the subsequent proteolytic lysis step take place in the Lysis Tube. After mechanical disruption and lysis of starting material the extraction procedure will be followed by the efficient binding of genomic DNA on a Spin Filter surface following washing of the bound DNA and finally eluting of the DNA. The recovery of DNA and the quality are excellent. The standard protocol for isolation of genomic DNA from a tissue sample will be finished within 30 minutes.

Description
Size
Unit

Order-No.

 
innuSPEED Tissue DNA Kit

For isolation of genomic DNA from different amounts (max. 50 mg) of tissue samples or biopsies.

10
50
250

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845-KS-1540010
845-KS-1540050
845-KS-1540250

 
innuSPEED Plant DNA Kit

For isolation of genomic DNA from different amounts (up to 100 mg) and different kinds of plant samples.

10
50
250

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845-KS-1560010
845-KS-1560050
845-KS-1560250

innuSPEED RNA Kits
For extreme fast and efficient isolation of total RNA from different starting materials. The kit contains specialized Lysis Tubes which will be processed in a homogenizer. The Lysis Tubes contain further an optimized Lysis Buffer which inactivate exogenous and endogenous RNases. The mechanical disruption of starting material and the subsequent sample Denaturation take place in the Lysis Tube. After mechanical disruption and denaturation of starting material the extraction procedure will be followed by the efficient removing of genomic DNA by binding on a first Spin Filter surface following binding of total RNA on a second Spin Filter surface following washing of the bound RNA and finally eluting of the RNA. The recovery of RNA and the quality are excellent. The standard protocol for isolation of total RNA from a plant sample will be finished within 20-30 minutes.

Description
Size
Unit

Order-No.

 
innuSPEED Tissue RNA Kit

Isolation of RNA from different amounts (up to 100 mg) and different kinds of tissue samples or biopsies.

10
50
250

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845-KS-2540010
845-KS-2540050
845-KS-2540250

 
innuSPEED Plant RNA Kit

For isolation of total RNA from different amounts (max. 100 mg) of different kinds of plant material.

10
50
250

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845-KS-2560010
845-KS-2560050
845-KS-2560250

 
innuPREP Lysis Tube A

Lysis Tube A is a 2.0 ml Tube with cap containing specialized beads for efficient disruption of plant or tissue samples using homogenizers.

10
50
250

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845-CS-1010050
845-CS-1010100
845-CS-1010500


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Costum-made Products   

Description
Size
Unit

Order-No.

 
innuSOLV RNA Reagent

Product for chemical extraction of total RNA. innuSOLV Reagent is a monophasic Solution for RNA isolation. After lysis of starting material proteins and genomic DNA will be removed by addition of chloroform and subsequent centrifugation based phase separation. Than the RNA will be precipitated from the aquous supernatant with alcohol. The innuSOLV Reagent is a very fast and efficient tool for isolation of pure RNA from different types and different quantities of starting materials.

10
100

ml
ml

845-SB-2090010
845-SB-2090100

 
Solution PPT

Solution PPT is a reagent for very fast and efficient precipitation of proteins from lysis preparations for isolation of DNA and RNA from different amounts and different types of starting materials.

100
500

ml
ml

845-SB-1090100
845-SB-1090500

 
INSTANT Viral RNA Kit-CE
For isolation of viral RNA from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs and from cell cultures using the Corbet Extractor (CE). The Kit contains all reqiered components as Filterplate, Depp Well Plate 2 ml, Extraction Tubes with carrier RNA and Internal Control.
196
480
Reactions
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845-SB-3090196
845-SB-3090480
 
INSTANT Viral DNA Kit-CE
For isolation of viral DNA from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs and from cell cultures using the Corbet Extractor (CE). The Kit contains all reqiered components as Filterplate, Depp Well Plate 2 ml, Extraction Tubes with carrier RNA and Internal Control.
196
480
Reactions
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845-SB-4090196
845-SB-4090480


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